In vivo MRI at mesoscopic resolution (0.1-0.5 mm) enables detailed visualization of the brains angioarchitecture, which can be examined across multiple spatial scales, including (1) leptomeningeal vessels, (2) pial vessels, and (3) intracortical vessels. Recently, using multi-shot, multi-echo 3D EPI with T2* contrast, we have achieved substantial advances in imaging the venous angioarchitecture in living humans at 0.35 mm isotropic resolution. Our optimized imaging protocol provides whole-brain coverage in under seven minutes, making the mesoscopic angioarchitecture imaging both feasible and practicable for a wide range of neuroimaging studies. Additionally, we have developed novel processing and analysis methods to enhance visualization and quantification of vascular structures across spatial scales. The combination of our imaging and analysis advancements open new opportunities for studying cerebrovascular function in relation to cortical layers and columns, as well as for anatomical investigations in developmental and clinical research.
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